Eric has it about right. If you want to keep everything intact, and just remove your data, do the following.
• First create a new admin account.
• Login to this account and delete all the other accounts.
• Delete anything else that you don’t want on the system from inside the Apps folder, or /Users/Shared.
• Delete everything from the root level of the hard drive leaving the default Applications, Library, System & Users folders. You can use a utility (like TinkerTool) to reveal hidden items if you’re really paranoid, but be careful. Some of the hidden items at the root level are part of the system.
• Launch Disk Utility, select your boot partition, and use the erase free space option. This will take a good deal of time for large drives, so be patient. The default single-pass option is fine.
At this point, your drive is clean of any personal data. If you’d like to have it boot as if it’s a new system with the Setup Assistant, there are a few extra steps. There’s a good article covering Leopard (w/ a link to the Tiger hint) over at OS X Hints.